Gammaglobulin electrophoresis

Definition

Serum immunoelectrophoresis is a test that tells whether or not you have immunoglobulins in the blood. Immunoglobulins are proteins that make antibodies. The proteins can be abnormal. There are various types of immunoglobulins. If you do have these proteins, this test can also help identify what specific type they are.

Alternative Names

IEP - serum; Immunoglobulin electrophoresis - serum; Gammaglobulin electrophoresis; Serum immunoglobulin electrophoresis

Why the Gammaglobulin electrophoresis is Performed

This test is performed to assess the clonality (monoclonal or polyclonal) of immunoglobulins.

How the Gammaglobulin electrophoresis is Performed

Blood is drawn from a vein, usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic. An elastic band is placed around the upper arm to apply pressure and cause the vein to swell with blood.

A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the band is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children, the area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. A bandage may be applied to the puncture site if there is any bleeding.

How the Gammaglobulin electrophoresis Will Feel

When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.

Risks

• Excessive bleeding
• Fainting or feeling lightheaded
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

What Abnormal Results Mean

In some malignant disorders (that is, multiple myeloma, chronic lymphocytic leukemia) a single clone of lymphocytes produces one type of protein -- a monoclonal immunoglobulin. This is identifiable as monoclonal (all the same type) by immunoelectrophoresis. Some people have monoclonal immunoglobulins, but do not have a malignant disorder.

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